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Has your wife been keeping a record of this? Can she start by maintaining a record and write down specific events, dates, times etc.
Thanks. What would be the next steps after recording all this?
Your wife needs to keep a daily diary of things said and done at work. Also, if there are witnesses to the unkind and unnecessary remarks from the manager, make a note of who witnessed. I'm wondering about the other 2 colleagues - are they forming a "team" with the manager against your wife? Do they make similar demoralizing comments to her? Do they appear to be siding with the manager's attitude? Does your wife have emotional support from anyone at the office? And who is at the top of the chain? Her Manager must report to someone.
Honestly, this sucks but the best thing would be for her to just find a new job in a new company. This kind of thing takes a toll on someone, and it almost always does not get better