I’d like to one day become a PI at a research university. What can I do to set myself up for that path as a first-time research assistant?

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To be the principal investigator is a long way, it is not easy but if you have the discipline I am sure you can do it. One thing that you cannot lack is to have leadership skills, I wish you luck on your way.

The first thing you must have is a doctorate or better a postdoc, the other thing to keep in mind is that it is a highly competitive world so you must be willing to let friends become rivals.

Trying to help you give you a good answer I found myself with an article on how to become a principal investigator, https://www.practicaladultinsights.com/how-do-i-become-a-principal-investigator.htm

It's very good.

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